Journal: Experimental and Therapeutic Medicine

Article Title: Pro‑angiogenic activity of salvianolate and its potential therapeutic effect against acute cerebral ischemia

doi: 10.3892/etm.2023.12108

Figure Lengend Snippet: Effect of Sal on the expression of VEGF mRNA and protein in macrophages. (A) Relative Vegf mRNA expression levels in RAW264.7 cells. (B) Levels of VEGF protein in the RAW264.7 cell supernatant. Values are presented as the mean ± SD (n=5). * P<0.05, ** P<0.01 vs. the control group. Sal, salvianolate; VEGF, vascular endothelial growth factor.

Article Snippet: Human umbilical vein endothelial cells (HUVECs; American Type Culture Collection HUV-EC-C cell line, cat. no. CRL-1730) were obtained from the Cancer Research Institute of Central South University.

Techniques: Expressing, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Pro‑angiogenic activity of salvianolate and its potential therapeutic effect against acute cerebral ischemia

doi: 10.3892/etm.2023.12108

Figure Lengend Snippet: Effect of Sal on the viability, cell cycle, migration and tube formation of HUVECs. (A) Effect of Sal on HUVEC viability. (B) Cell cycle distribution of the HUVECs was detected by flow cytometry and (C) the proportion of cells in the S phase was quantified. (D) Representative images from a wound healing assay of HUVECs at 0 and 24 h (scale bar, 200 µm) and (E) quantitative analysis of cell migration to the wound. (F) Quantitative analysis of tube formation and (G) representative images of tube formation (scale bar, 200 µm). Values are presented as the mean ± SD (n=3 per group); * P<0.05, ** P<0.01, *** P<0.001 vs. the control group; # P<0.05, ## P<0.01, & P<0.05, && P<0.01 as indicated. Sal, salvianolate; HUVECs, human umbilical vein endothelial cells; VEGF, vascular endothelial growth factor. Control group, untreated HUVECs; s-control group, HUVECs treated with supernatant of RAW264.7 cells; Sal group, HUVECs treated with 10 µM Sal; s-Sal group, HUVECs treated with supernatant of Sal (10 µM)-treated RAW264.7 cells; VEGF group, HUVECs treated with 10 ng/ml VEGF as a positive control.

Article Snippet: Human umbilical vein endothelial cells (HUVECs; American Type Culture Collection HUV-EC-C cell line, cat. no. CRL-1730) were obtained from the Cancer Research Institute of Central South University.

Techniques: Migration, Flow Cytometry, Wound Healing Assay, Control, Positive Control

Journal: Experimental and Therapeutic Medicine

Article Title: Pro‑angiogenic activity of salvianolate and its potential therapeutic effect against acute cerebral ischemia

doi: 10.3892/etm.2023.12108

Figure Lengend Snippet: Effect of Sal on the VEGF signaling pathway in vitro . (A) Representative western blots of VEGFR-2, p-VEGFR-2, AKT, p-AKT, p38, p-p38 and β-actin. Quantitative analysis of the relative levels of (B) p-VEGFR-2, (C) p-AKT and (D) p-p38 normalized to those of total VEGFR-2, AKT and p38, respectively. Values are presented as the mean ± SD (n=3 per group); * P<0.05, ** P<0.01, *** P<0.001 vs. the control group; ## P<0.01, & P<0.05, && P<0.01 as indicated. Sal, salvianolate; VEGF, vascular endothelial growth factor; VEGFR-2, VEGF receptor 2; p-, phosphorylated; Control group, untreated HUVECs; s-control group, HUVECs treated with supernatant of RAW264.7 cells; Sal group, HUVECs treated with 10 µM Sal; s-Sal group, HUVECs treated with supernatant of Sal (10 µM)-treated RAW264.7 cells; VEGF group, HUVECs treated with 10 ng/ml VEGF as a positive control.

Article Snippet: Human umbilical vein endothelial cells (HUVECs; American Type Culture Collection HUV-EC-C cell line, cat. no. CRL-1730) were obtained from the Cancer Research Institute of Central South University.

Techniques: In Vitro, Western Blot, Control, Positive Control

Journal: Experimental and Therapeutic Medicine

Article Title: Pro‑angiogenic activity of salvianolate and its potential therapeutic effect against acute cerebral ischemia

doi: 10.3892/etm.2023.12108

Figure Lengend Snippet: Effect of Sal on the histopathology and protein expression of VEGF and VEGFR-2 in a rat model of transient middle cerebral artery occlusion. (A) H&E-stained cerebral cortex and striatum (scale bar, 100 µm). (B) Representative immunohistochemical images of (B) VEGF and (C) VEGFR-2 staining (scale bar, 100 µm). Quantitative analysis showing the integrated intensity of (D) VEGF and (E) VEGFR-2 staining. Values are presented as the mean ± SD (n=5 per group). ** P<0.01, *** P<0.001 vs. the sham group; # P<0.05, vs. the infarct group. Sal, salvianolate; VEGF, vascular endothelial growth factor; VEGFR-2, VEGF receptor 2; ED, edaravone.

Article Snippet: Human umbilical vein endothelial cells (HUVECs; American Type Culture Collection HUV-EC-C cell line, cat. no. CRL-1730) were obtained from the Cancer Research Institute of Central South University.

Techniques: Histopathology, Expressing, Staining, Immunohistochemical staining

Journal: Experimental and Therapeutic Medicine

Article Title: Pro‑angiogenic activity of salvianolate and its potential therapeutic effect against acute cerebral ischemia

doi: 10.3892/etm.2023.12108

Figure Lengend Snippet: Cellular location and expression of VEGF and VEGFR-2 proteins in the brain tissues of rats with transient middle cerebral artery occlusion. (A) Representative immunofluorescence double staining images of VEGF and F4/80 (scale bar, 100 µm) and (B) the intensity of VEGF immunofluorescence. (C) Representative immunofluorescence double staining images of VEGFR-2 and CD31 (scale bar, 100 µm) and (D) the intensity of VEGFR-2 immunofluorescence. Quantification was performed using ImageJ software. Values are presented as the mean ± SD (n=5 per group). * P<0.05 vs. the sham group; # P<0.05, ## P<0.01 vs. the infarct group; %% P<0.01 as indicated. VEGF, vascular endothelial growth factor; VEGFR-2, VEGF receptor 2; Sal, salvianolate; ED, edaravone.

Article Snippet: Human umbilical vein endothelial cells (HUVECs; American Type Culture Collection HUV-EC-C cell line, cat. no. CRL-1730) were obtained from the Cancer Research Institute of Central South University.

Techniques: Expressing, Immunofluorescence, Double Staining, Software

Journal: Experimental and Therapeutic Medicine

Article Title: Pro‑angiogenic activity of salvianolate and its potential therapeutic effect against acute cerebral ischemia

doi: 10.3892/etm.2023.12108

Figure Lengend Snippet: Effect of Sal on the VEGF signaling pathway in a rat model of transient middle cerebral artery occlusion. (A) Representative western blots of VEGF, VEGFR-2, p-VEGFR-2, p-AKT, AKT, p38, p-p38 and β-actin. Quantitative analysis of the relative levels of (B) VEGF and (C) VEGFR-2 normalized to those of β-actin, (D) p-VEGFR-2 relative to those of total VEGFR-2, (E) p-AKT relative to those of total AKT and (F) p-p38 relative to those of total p38. Values are presented as the mean ± SD (n=3 per group). * P<0.05 vs. the sham group; # P<0.05, ## P<0.01 vs. the infarct group; && P<0.01 as indicated. Sal, salvianolate; VEGF, vascular endothelial growth factor; VEGFR-2, VEGF receptor 2; p-, phosphorylated; ED, edaravone.

Article Snippet: Human umbilical vein endothelial cells (HUVECs; American Type Culture Collection HUV-EC-C cell line, cat. no. CRL-1730) were obtained from the Cancer Research Institute of Central South University.

Techniques: Western Blot

Journal: Experimental and Therapeutic Medicine

Article Title: Pro‑angiogenic activity of salvianolate and its potential therapeutic effect against acute cerebral ischemia

doi: 10.3892/etm.2023.12108

Figure Lengend Snippet: Schematic diagram of the proposed molecular mechanisms underlying the protective effects of Sal treatment against cerebral ischemia-reperfusion injury, which involves macrophage activation-induced HUVEC angiogenesis. Sal, salvianolate; HUVECs, human umbilical vein endothelial cells; VEGF, vascular endothelial growth factor; VEGFR-2, VEGF receptor 2; p, phosphorylated.

Article Snippet: Human umbilical vein endothelial cells (HUVECs; American Type Culture Collection HUV-EC-C cell line, cat. no. CRL-1730) were obtained from the Cancer Research Institute of Central South University.

Techniques: Activation Assay